Anti-Human ENG Recombinant Antibody (TAB-232CL)
CAT#: TAB-232CL
Recombinant Chimeric (human/mouse) monoclonal antibody expressed in CHO binding to Human ENG. This antibody is a novel antibody to endoglin, which is a protein that is overexpressed on endothelial cells and is essential for angiogenesis, the process of new blood vessel formation.
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Anti-Human ENG Recombinant Antibody (TAB-232CL) in SDS-PAGE
Lane 1: 2 μg, Reducing mAb Lane M: Protein Marker
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/1823_F9_38_selected.jpg
Subcellular Location
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows moderate membranous positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11862/158146_A_2_7_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nuclear bodies & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/1777_G1_13_cr59632eeaa8e82_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nuclear bodies & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/1759_C2_13_cr594b6f97edc10_blue_red_green.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/156475_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (fHDF/TERT166, HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000106991-ENG
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Specifications
- Host Species
- mouse
- Type
- Antibody
- Species Reactivity
- Human
- Related Disease
- Solid Tumors
Product Property
- Purity
- Purity>95% by SDS-PAGE
- Storage
- ≥1 year at -20°C. If the reconstituted antibody cannot be used within two weeks, it should be aliquoted into smaller vials and stored at -20°C
Target
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Fantastic Research Option
In our laboratory, this antibody has proven to be a reliable product. It has consistently performed well across a wide range of assays.
Increased Binding Efficiency
It is useful for activities requiring precision, such as measuring the target chemical at low concentrations.
Cost-Effective
Despite cheap, this antibody performs well. It meets our research needs while remaining within budget, which is critical for project funding.
Q&As
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How can I confirm antibody binding specificity in my sample?
A: Perform a titration series, use various controls, and conduct parallel assays with different antibodies targeting known concentrations or expressions of the protein of interest.
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How can I optimize the blocking step in my protocols?
A: Test different blocking agents like BSA, serum, or commercial blocking buffers and adjust concentrations and incubation times to reduce background and enhance signal specificity.
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What methods are available for conjugating the antibody with detection agents?
A: Chemical cross-linking kits for labeling antibodies with enzymes, fluorophores, or other tags are commercially available to aid in detecting the antibody.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-232CL, RRID: AB_3111891)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ENG"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1569CL | Human Anti-ENG Recombinant Antibody (TAB-1569CL) | ELISA, FC | Human antibody |
| TAB-1571CL | Human Anti-ENG Recombinant Antibody (TAB-1571CL) | ELISA, FC | Human IgG1 |
| TAB-1577CL | Anti-Human ENG Recombinant Antibody (9H10) | FuncS | Fully human antibody |
| TAB-1569CL-S(P) | Human Anti-ENG Recombinant Antibody; scFv Fragment (TAB-1569CL-S(P)) | ELISA, FC | Human scFv |
| TAB-1571CL-S(P) | Human Anti-ENG Recombinant Antibody; scFv Fragment (TAB-1571CL-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1573CL | Mouse Anti-ENG Recombinant Antibody (TAB-1573CL) | FC, Apop | Mouse IgG |
| TAB-1574CL | Mouse Anti-ENG Recombinant Antibody (TAB-1574CL) | FC | Mouse IgG |
| TAB-1573CL-S(P) | Mouse Anti-ENG Recombinant Antibody; scFv Fragment (TAB-1573CL-S(P)) | FC, Apop | Mouse scFv |
| TAB-1574CL-S(P) | Mouse Anti-ENG Recombinant Antibody; scFv Fragment (TAB-1574CL-S(P)) | FC | Mouse scFv |
| TAB-1573CL-F(E) | Mouse Anti-ENG Recombinant Antibody; Fab Fragment (TAB-1573CL-F(E)) | FC, Apop | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1575CL | Human Anti-ENG Recombinant Antibody (TAB-1575CL) | Apop | Humanized antibody |
| TAB-1576CL | Human Anti-ENG Recombinant Antibody (TAB-1576CL) | Cyt, ELISA, FC, FuncS | Humanized antibody |
| TAB-1575CL-S(P) | Human Anti-ENG Recombinant Antibody; scFv Fragment (TAB-1575CL-S(P)) | Apop | Human scFv |
| TAB-1576CL-S(P) | Human Anti-ENG Recombinant Antibody; scFv Fragment (TAB-1576CL-S(P)) | Cyt, ELISA, FC, FuncS | Human scFv |
| TAB-1575CL-F(E) | Human Anti-ENG Recombinant Antibody; Fab Fragment (TAB-1575CL-F(E)) | Apop | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0189MZ | Chicken Anti-Endoglin Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-726CQ | Mouse Anti-ENG Recombinant Antibody (clone 7D11) | WB, Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1150 | Hi-Affi™ Rabbit Anti-ENG Recombinant Antibody (clone DS1150AB) | WB, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-013ML | Anti-Human ENG Recombinant Antibody (TAB-013ML) | ELISA, IHC, FC, IP, IF, Inhib | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0231-YC-S(P) | Human Anti-ENG Recombinant Antibody (clone Vκ2VH1); scFv Fragment | ELISA, WB, FuncS | Humanized scFv |
| HPAB-0713-CN-S(P) | Human Anti-ENG Recombinant Antibody (clone 6B10); scFv Fragment | WB, FC | Human scFv |
| HPAB-AP642-YC-S(P) | Human Anti-ENG Recombinant Antibody (clone Vκ1VH1); scFv Fragment | ELISA | Humanized scFv |
| HPAB-AP644-YC-S(P) | Human Anti-ENG Recombinant Antibody (clone Vκ2VH2); scFv Fragment | ELISA | Humanized scFv |
| HPAB-J0071-YC-S(P) | Mouse Anti-ENG Recombinant Antibody (clone AP020/YC); scFv Fragment | ELISA, WB, ICC, IHC, IF, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0231-YC-F(E) | Human Anti-ENG Recombinant Antibody (clone Vκ2VH1); Fab Fragment | ELISA, WB, FuncS | Humanized Fab |
| HPAB-0162CQ-F(E) | Mouse Anti-ENG Recombinant Antibody (clone E9); Fab Fragment | ELISA, WB, FC, IHC, IHC-Fr, IP | Mouse Fab |
| HPAB-0137-WJ-F(E) | Human Anti-ENG Recombinant Antibody; Fab Fragment (HPAB-0137-WJ-F(E)) | ELISA | Humanized Fab |
| HPAB-AP642-YC-F(E) | Human Anti-ENG Recombinant Antibody (clone Vκ1VH1); Fab Fragment | ELISA | Humanized Fab |
| HPAB-AP644-YC-F(E) | Human Anti-ENG Recombinant Antibody (clone Vκ2VH2); Fab Fragment | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0713-CN | Human Anti-ENG Recombinant Antibody (clone 6B10) | WB, FC | Human IgG |
| HPAB-0714-CN | Human Anti-ENG Recombinant Antibody (clone 4.120) | WB, FC | Human IgG |
| HPAB-0162CQ | Mouse Anti-ENG Recombinant Antibody (clone E9) | ELISA, WB, FC, IHC, IHC-Fr, IP | Mouse IgG1 |
| ZG-0076J | Mouse Anti-ENG Recombinant Antibody (ZG-0076J) | IHC-P, IF | Mouse IgG |
| ZG-0077J | Mouse Anti-ENG Recombinant Antibody (ZG-0077J) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-013ML | Afuco™ Anti-ENG ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-013ML) | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0613 | PE-A*02:01/Human ENG (LLTAALWYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0614 | APC-A*02:01/Human ENG (LLTAALWYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0615 | FITC-A*02:01/Human ENG (LLTAALWYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0616 | A*02:01/Human ENG (LLTAALWYV) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0707-YJ-VHH | Camelid Anti-ENG Recombinant Single Domain Antibody (HPAB-0707-YJ-VHH) | ELISA, ICC | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1247 | AbPlus™ Anti-ENG Magnetic Beads (VS-0724-YC1247) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY186 | Mouse Anti-NHP ENG Recombinant Antibody (clone ENG/1621) | IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY103 | CytoStream™ Mouse Anti-ENG Recombinant Antibody (VS-0225-XY103) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY146 | Human Anti-ENG (clone 6B10) scFv-Fc Chimera | WB, FC, ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC237 | Recombinant Anti-ENG Vesicular Antibody, EV Displayed (VS-0425-YC237) | Cyt, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2282 | Anti-Mouse ENG Immunohistochemistry Kit | IHC |
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